Anaerobic Acidogenic Fermentation of Cellobiose by Immobilized Cells: Prediction of Organic Acids Production by Response Surface Methodology

Response surface methodology was used to derive a prediction model for organic acids production by anaerobic acidogenic fermentation of cellobiose, using mixed culture immobilized on γ-alumina. Three parameters (substrate concentration, temperature, and initial pH) were evaluated. In order determine the limits parameters, preliminary experiments at 37 °C conducted substrates various cellobiose concentrations pH values. Cellobiose as sugar subsequent with lignocellulosic biomass. The well adapted successive subculturing in synthetic media (with 100:5:1 COD:N:P ratio). experimental data batch fermentations fitted into polynomial total concentration predictive that could be utilized tool predict results when biomass is substrate. quadratic effect temperature most significant, followed linear concentration. corroborated validity effectiveness model.